The paper aims to identify the scope and propose a systematisation of the contemporary redefinition of regional development factors, influenced by global conditions. The research process comprised three stages: (1) a brief review of the theoretical foundations and changes in the understanding of regional development factors; (2) presentation of the findings relating to the directions and impact of contemporary socio-economic megatrends on changes of regional development factors, and (3) the main body of the analysis, which identifies and describes the nature of changes in the perception of regional development factors as a consequence of contemporary global conditions.