Competitiveness is a key factor determining the development of a region and hence the standard of living of its inhabitants, and human capital is one of the most important factors enabling improvement of regional competitiveness. The aim of the presented research is to analyse the spatial differentiation of the level of human capital in the regions of the European Union (EU) in relation to their level of competitiveness. The results show significant disproportions in this respect, especially between the so-called old and new EU. The higher levels of human capital in the old EU regions were also generally accompanied by a higher level of competitiveness. In the long run, this will lead to an increase in regional disparities in development.