Financial resources from the European Union can be an important factor in the development of various sectors of the economy, including transport. Among other things, they are used to make investments in transport infrastructure and in elements related to the operation of sustainable and integrated urban transport. The conducted study concerns the identification of the scope of the use of EU funds by cities and other entities operating urban transport, as well as tasks related to the implementation of the assumptions of sustainable transport in the years 2014–2020. The study covers Polish cities that have benefited from the European Union’s subsidies in this regard. The results of the survey allowed us to observe certain trends in the implementation of investments, which are related to the size of urban units, such as the dependence of the type of investment on the size of the city. The smaller the city, the more investments related to the construction of an interchange or interchange centre and fewer investments related to the construction of bicycle paths and roads.